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Yeasen Biotechnology superoxide red fluorescent probe
Superoxide Red Fluorescent Probe, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superoxide+red+fluorescent+probe/indicator+mitochondrial+mitosox+red+superoxide/pm41944296-230-2-21
Average 86 stars, based on 1 article reviews
superoxide red fluorescent probe - by Bioz Stars, 2026-10
86/100 stars

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Membrane:

Article Title: Brain and Liver Dual-Targeting Oridonin Nanoparticles to Enhance Aβ Clearance for Alzheimer's Disease Therapy.
Article Snippet: RIPA lysis buffer, BCA protein assay kit, SDS-PAGE sample loading buffer (5 ×), and ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). .. The mitochondrial superoxide red fluorescent probe, mitochondrial membrane permeability transition pore detection kit, and mitochondrial green fluorescent probe were obtained from Yeasen Biotechnology Co., Ltd. (Shanghai, China). ..

Permeability:

Article Title: Brain and Liver Dual-Targeting Oridonin Nanoparticles to Enhance Aβ Clearance for Alzheimer's Disease Therapy.
Article Snippet: RIPA lysis buffer, BCA protein assay kit, SDS-PAGE sample loading buffer (5 ×), and ROS assay kit were purchased from Beyotime Biotechnology (Shanghai, China). .. The mitochondrial superoxide red fluorescent probe, mitochondrial membrane permeability transition pore detection kit, and mitochondrial green fluorescent probe were obtained from Yeasen Biotechnology Co., Ltd. (Shanghai, China). ..



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IR combined with BIBR1532 enhanced <t>mitochondrial</t> damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red <t>fluorescent</t> polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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IR combined with BIBR1532 enhanced <t>mitochondrial</t> damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red <t>fluorescent</t> polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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IR combined with BIBR1532 enhanced <t>mitochondrial</t> damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red <t>fluorescent</t> polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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IR combined with BIBR1532 enhanced <t>mitochondrial</t> damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red <t>fluorescent</t> polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
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IR combined with BIBR1532 enhanced <t>mitochondrial</t> damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red <t>fluorescent</t> polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Mitosox Red Mitochondrial Superoxide Indicator Fluorescent Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


IR combined with BIBR1532 enhanced mitochondrial damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red fluorescent polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Journal: Journal of Translational Medicine

Article Title: BIBR1532 combined with radiotherapy induces ferroptosis in NSCLC cells and activates cGAS-STING pathway to promote anti-tumor immunity

doi: 10.1186/s12967-024-05331-3

Figure Lengend Snippet: IR combined with BIBR1532 enhanced mitochondrial damage-induced mtDNA release. A Representative fluorescence images of mROS in NSCLC cells following different treatments. Scale bar = 20 μm. B JC-1 dye was employed to monitor changes in the MMP of NSCLC cells after various treatments. Healthy mitochondria display red fluorescent polymers (J-aggregates), while a reduction in mitochondrial membrane potential results in the green fluorescent monomer form. Scale bar = 50 μm. C TEM images of H460 and A549 cells pre-treated with BIBR1532 without irradiation (control) or 24 h after 6 Gy irradiation. (Mitochondria; red arrows, Scale bars: top, 5 μm; middle, 2 μm; below, 1 μm). D, E Quantitative analysis of mitochondrial DNA and cDNA in H460 and A549 cells using qRT-PCR. F The DNA oxidative damage biomarker 8-OHDG (green) and mitochondrial marker protein TOMM20 (red) were employed to assess mtDNA damage of H460 cells after various treatments. The data is presented as mean ± standard deviation, and each experiment was independently repeated three times. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: After irradiation treatment, a 5 μM MitoSOX RED (40778ES50, Yeasen Biotechnology) mitochondrial superoxide red fluorescent probe was added.

Techniques: Fluorescence, Membrane, Irradiation, Control, Quantitative RT-PCR, Biomarker Discovery, Marker, Standard Deviation